Development of hematopoietic repopulating cells from embryonic stem cells.
نویسندگان
چکیده
A reliable and effective in vitro differentiation system is described for the production of functional neutrophils from mouse embryonic stem (ES) cells. A three-step culture method was developed that enables abundant production and effective harvesting of mature neutrophils at high purity without sorting. Utilization of the OP9 stromal cell line, which does not produce macrophage colony stimulating factor (M-CSF) was found to enhance the number, percentage and duration of neutrophils produced. Based on a number of criteria, morphologically and functionally mature [8] DIFFERENTIATION OF ES CELLS INTO NEUTROPHILS 129 Copyright 2003, Elsevier Inc. All rights reserved. METHODS IN ENZYMOLOGY, VOL. 365 0076-6879/2003 $35.00
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ورودعنوان ژورنال:
- Methods in enzymology
دوره 365 شماره
صفحات -
تاریخ انتشار 2003